Colorectal cancer (CRC) ranks third globally in terms of prevalence, accounting for 10% of cancer cases and deaths each year. Irreversible electroporation (IRE) offers promise for treating CRC by selectively destroying tumor cells while minimizing harm to healthy tissue. However, the lack of effective guidance and evaluation methods hampers the achievement of complete tumor ablation. To address this, integrated OCT, US, and NIRF endoscopy enable real-time imaging of colorectal tumors, facilitating precise IRE treatment and evaluation of outcomes. In this study, we used an integrated imaging system to precisely guide and evaluate the implementation of IRE therapy in CRC.
Optical coherence tomography (OCT) is an indispensable diagnostic tool in ophthalmology which can provide crosssectional anatomic and functional information of the eyes. For analysis of the optical imaging performance of the eye, the ZEMAX ray tracing software can be used to establish the refractive model and to simulate the light spot and wavefront in ocular fundus. In our study, by combination of OCT and ray tracing technique, the imaging performance of mouse eye was evaluated and the central and peripheral image qualities were quantitatively analyzed. The whole mouse eyes were imaged by a customized OCT system and the OCT images were corrected for distortions caused by the refraction of light on the ocular surface. Parameters of mouse eye, both at central and peripheral areas, including corneal thickness, radius of curvature, lens thickness, etc., were then measured. Finally, the imaging performance of the ophthalmology system was evaluated by using ZEMAX software for ray tracing. The light spot size, the defocus blur parameter and the wavefront aberration were quantitatively evaluated. Our study may provide a method for research on myopia and hyperopia.
Optical coherence tomography (OCT) is a noninvasive, non-contact imaging technique, which is an indispensable diagnostic tool in ophthalmology. However, light attenuation in opaque tissues of the eye limits the imaging depth of OCT. In this study, the imaging depth of rabbit eye in vitro was extended by reduction of multiple scattering by using tissue optical clearing (TOC) agents. Results showed that with the application of glycerol in anterior segment, boundaries of ciliary muscle could be obviously differentiated. For posterior segment, the retina, the choroid, the sclera and even the eye container were also clearly imaged. All the physiology change by glycerol was recovered by a direct wash of saline in a short time.
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